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KMID : 0880220090470040506
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Journal of Microbiology
2009 Volume.47 No. 4 p.506 ~ p.513
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Identification of a novel Streptomyces chattanoogensis L10 and enhancing its natamycin production by overexpressing positive regulator ScnRII
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Du Yi-Ling
Chen Shi-Fei
Cheng Liang-Ying
Shen Xue-Ling
Tian Yuan
Li Yong-Quan
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Abstract
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A novel Streptomyces strain, L10, which is capable of producing natamycin, was isolated from a soil sample collected from Zhejiang province, China. On the basis of phylogenetic analysis of rpoB gene and 16S rDNA sequences, as well as phenotypic comparison, strain L10 (CGMCC 2644) is proposed to be a previously uncharacterized strain of S. chattanoogensis. By screening a cosmid library of strain L10 and primer walking, a partial sequence of scnRI and the entire sequence of scnRII were obtained, which are orthologues to the pathway-specific positive regulator genes of natamycin biosynthesis in S. natalensis. The engineered S. chattanoogensis Dl, generated by inserting an additional copy of scnRII into the chromosome of strain L10, increased its natamycin production by 3.3 fold in YSG medium and 4.6 fold in YEME medium without sucrose.
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KEYWORD
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Streptomyces chattanoogensis L10, natamycin, pathway-specific regulatory genes, strain improvement, genetic engineering
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